Targeted Delivery of Dendritic Polyglycerol鈥揇oxorubicin Conjugates by scFv-SNAP Fusion Protein Suppresses EGFR+ Cancer Cell Growth

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• 作者：Ahmad Fawzi Hussain ; Harald Rune Kr眉ger ; Florian Kampmeier ; Tim Weissbach ; Kai Licha ; Felix Kratz ; Rainer Haag ; Marcelo Calder贸n ; Stefan Barth
• 刊名：Biomacromolecules
• 出版年：2013
• 出版时间：August 12, 2013
• 年：2013
• 卷：14
• 期：8
• 页码：2510-2520
• 全文大小：491K
• 年卷期：v.14,no.8(August 12, 2013)
• ISSN：1526-4602

文摘

Development of effective polymer-based nanocarriers for the successful application in cancer therapy still remains a great challenge in current research. In the present study we present a dendritic polyglycerol-based multifunctional drug immunoconjugate that specifically targets and kills cancer cell lines expressing epidermal growth factor receptor (EGFR). The nanocarrier was provided with a dendritic core as a multifunctional anchoring point, doxorubicin (Doxo) coupled through a pH-sensitive linker, a fluorescence marker, poly(ethylene glycol), as solubilizing and shielding moiety, and a scFv antibody conjugated through the SNAP-Tag technology. The study provides the proof of principle that SNAP-tag technology can be used to generate drug-carrying nanoparticles efficiently modified with single-chain antibodies to specifically target and destroy cancer cells.