Altering Colloidal Surface Functionalization Using DNA Encapsulated Inside Monodisperse Gelatin Microsphere Templates

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• 作者：James O. Hardin ; Alberto Fernandez-Nieves ; Carlos J. Martinez ; Valeria T. Milam
• 刊名：Langmuir
• 出版年：2013
• 出版时间：May 7, 2013
• 年：2013
• 卷：29
• 期：18
• 页码：5534-5539
• 全文大小：286K
• 年卷期：v.29,no.18(May 7, 2013)
• ISSN：1520-5827

文摘

Soluble oligonucleotides are typically introduced to bulk solution to promote hybridization activity on DNA-functionalized surfaces. Here, an alternative approach is explored by encapsulating secondary target strands inside semipermeable colloidal satellite assemblies, then triggering their release at 37 掳C for subsequent surface hybridization activity. To prepare DNA-loaded satellite assemblies, uniform gelatin microspheres are fabricated using microfluidics, loaded with 15 base-long secondary DNA targets, capped with a polyelectrolyte bilayer, and finally coated with a monolayer of polystyrene microspheres functionalized with duplexes comprised of immobilized probes and soluble, 13 base-long hybridization partner strands. Once warmed to 37 掳C, secondary DNA targets are released from the gelatin template and then competitively displace the shorter, original hybridization partners on the polystyrene microspheres.