Detection of Intact Influenza Viruses using Biotinylated Biantennary S-Sialosides

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• 作者：Ramesh R. Kale ; Harshini Mukundan ; Dominique N. Price ; J. Foster Harris ; Daniel M. Lewallen ; Basil I. Swanson ; Jurgen G. Schmidt ; Suri S. Iyer
• 刊名：Journal of the American Chemical Society
• 出版年：2008
• 出版时间：July 2, 2008
• 年：2008
• 卷：130
• 期：26
• 页码：8169 - 8171
• 全文大小：764K
• 年卷期：v.130,no.26(July 2, 2008)
• ISSN：1520-5126

文摘

We report the modular synthesis of robust, biotinylated biantennary sialylglycoconjugates and their ability to differentiate between two type A influenza strains. This is the first demonstration of glycoconjugate-based discriminatory capture and detection of two strains of intact influenza virus, in the presence of the innate enzymatic activity of viral neuraminidases. We also demonstrate a “carboassay” using glycoconjugates as capture and reporter elements, which therefore, does not require antibodies. The capture of intact influenza viruses is of potential benefit for clinical diagnostics.