Evolving Trends in the Dereplication of Natural Product Extracts: New Methodology for Rapid, Small-Scale Investigation of Natural Product Extracts

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• 作者：Gerhard Lang ; Nor Ainy Mayhudin ; Maya I. Mitova ; Lin Sun ; Sonia van der Sar ; John W. Blunt ; Anthony L. J. Cole ; Gill Ellis ; Hartmut Laatsch ; Murray H. G. Munro
• 刊名：Journal of Natural Products
• 出版年：2008
• 出版时间：September 26, 2008
• 年：2008
• 卷：71
• 期：9
• 页码：1595-1599
• 全文大小：222K
• 年卷期：v.71,no.9(September 26, 2008)
• ISSN：1520-6025

文摘

The use of an HPLC bioactivity profiling/microtiter plate technique in conjunction with capillary probe NMR instrumentation and access to appropriate databases effectively short-circuits conventional dereplication procedures, necessarily based on multimilligram extracts, to a single, more rapid submilligram operation. This approach to dereplication is illustrated using fungal or bacterial extracts that contain known compounds. In each case the dereplication steps were carried out on microgram quantities of extract and demonstrate the discriminating power of ¹H NMR spectroscopy as a definitive dereplication tool.