Adsorption of Laminin on Hydroxyapatite and Alumina and the MC3T3-E1 Cell Response

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• 作者：Hiroka Fujita ; Tada-aki Kudo ; Hiroyasu Kanetaka ; Toshiki Miyazaki ; Masami Hashimoto ; Masakazu Kawashita
• 刊名：ACS Biomaterials Science & Engineering
• 出版年：2016
• 出版时间：July 11, 2016
• 年：2016
• 卷：2
• 期：7
• 页码：1162-1168
• 全文大小：391K
• ISSN：2373-9878

文摘

Artificial hydroxyapatite (HAp) is osteoconductive, but the mechanism is still unclear. It is likely that some serum proteins are adsorbed onto HAp and influence its osteoconductivity. We investigated the adsorption behavior of laminin (LN), which was isolated from murine Engelbreth–Holm–Swarm sarcoma, onto HAp and compared it with nonosteoconductive alpha-type alumina (α-Al₂O₃). Cell adhesion, spreading, and proliferation on native and LN-adsorbed discs of HAp or α-Al₂O₃ were examined using murine MC3T3-E1 osteoblastic cells. A larger amount of LN adsorbed onto HAp than α-Al₂O₃ despite the electrostatic repulsion between LN and HAp, suggesting the specific adsorption of LN onto HAp. The LN adsorbed onto HAp remarkably enhanced initial attachment and spreading of MC3T3-E1 cells, but subsequent proliferation of MC3T3-E1 cells was influenced by the type of material rather than LN adsorption. These fundamental findings imply that LN adsorbed on HAp could trigger osteoconductivity in vivo, aiding in the development of novel biomaterials that specifically adsorb LN and effectively enhance cell attachment and spreading.