Very High-Field EPR Study of Glycyl Radical Enzymes

详细信息    查看全文

• 作者：Carole Duboc-Toia ; Alia K. Hassan ; Etienne Mulliez ; Sandrine Ollagnier-de Choudens ; Marc Fontecave ; Christina Leutwein ; and Johann Heider
• 刊名：Journal of the American Chemical Society
• 出版年：2003
• 出版时间：January 8, 2003
• 年：2003
• 卷：125
• 期：1
• 页码：38 - 39
• 全文大小：44K
• 年卷期：v.125,no.1(January 8, 2003)
• ISSN：1520-5126

文摘

This work shows that very high-field EPR spectroscopy allows a rather accurate determination of the g-tensor of protein radicals, including C-centered ones, and thus may be used as a probe for distinguishing a tyrosyl-, a glycyl-, or a tryptophanyl-radical. In this paper, we report the first complete analysis of the g-tensor of glycyl radical enzymes (anaerobic ribonucleotide reductase, pyruvate formate lyase, and benzylsuccinate synthase), thus providing new information on their EPR properties. Because the g-anisotropy is small, the complete resolution of the g-tensor could be only obtained at very high field (18.8 T).