Identification and Characterization of the Precursor of Chicken Matrix Metalloprotease 2 (pro-MMP-2) in Hen Egg

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• 作者：Sophie Ré ; hault-Godbert ; Joë ; l Gautron ; Valé ; rie Labas ; Maya Belghazi ; Yves Nys
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2008
• 出版时间：August 13, 2008
• 年：2008
• 卷：56
• 期：15
• 页码：6294-6303
• 全文大小：334K
• 年卷期：v.56,no.15(August 13, 2008)
• ISSN：1520-5118

文摘

Using zymography and mass spectrometry, we identified for the first time the precursor of chicken matrix metalloprotease 2 (pro-MMP-2) as a complex with TIMP-2 (tissue inhibitor of metalloproteinases) in egg white and yolk. Real-time polymerase chain reaction confirmed that MMP-2 and its inhibitors TIMP-2 and TIMP-3 were expressed all along the oviduct and in the liver of laying hens. We also demonstrated that the processing of pro-MMP-2 into mature MMP-2 by serine proteases does not occur in vivo, although purified pro-MMP-2 undergoes proteolytic maturation by these proteases in vitro. Moreover, the relative pro-MMP-2 activity assessed by gelatin zymography was shown to decrease in egg white during the storage of unfertilized or fertilized eggs. However, the mature form of 62 kDa MMP-2 could not be detected. The fact that MMP-2 is found as a proform in fresh eggs suggests that the activity of this metalloprotease is regulated under specific conditions during embryonic development.