The characteristics of dried residues of picodroplets ofsingle-, two-, and three-element aqueous solutions, whichqualify these as reference materials in the direct analysisof single particles, single cells, and other microscopicobjects using, e.g., laser ablation inductively coupledplasma time-of-flight mass spectrometry (LA-ICP-TOF-MS) and micro-X-ray fluorescence (MXRF), were evaluated. Different single-, two-, and three-element solutions(0.01-1 g/L) were prepared in picoliter volume (around130 pL) with a thermal inkjet printing technique. Anachievable dosing precision of 4-15% was calculated bytotal reflection X-ray fluorescence (TXRF) determinationof the transferred elemental mass of an array of 100droplets. The size of the dried residues was determinedby optical microscopy to be 5-20
m in diameter depending on the concentration and the surface material.The elemental distribution of the dried residues wasdetermined with synchrotron micro-X-ray fluorescence(SR-MXRF) analyses. The MXRF results show high uniformity for element deposition of every single droplet withan RSTD of 4-6% depending on the concentration ofspotted solution. The shape and height profile of driedresidues from picoliter droplets were studied using atomicforce microscopy (AFM). It was found that these dry togive symmetrical spherical segments with maximumheights of 1.7
m. The potential of this technique fordirect LA-ICP-TOF-MS analysis is shown.