Sol鈥揋el Derived Nanoporous Compositions for Entrapping Small Molecules and Their Outlook toward Aptamer Screening
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- 作者：Ji-Young Ahn ; SangWook Lee ; Minjoung Jo ; Jeehye Kang ; Eunkyung Kim ; Ok Chan Jeong ; Thomas Laurell ; Soyoun Kim
- 刊名：Analytical Chemistry
- 出版年：2012
- 出版时间：March 20, 2012
- 年：2012
- 卷：84
- 期：6
- 页码：2647-2653
- 全文大小：401K
- 年卷期：v.84,no.6(March 20, 2012)
- ISSN：1520-6882
文摘
This paper reports for the first time the application of sol鈥揼el microarrays for immobilizing nonsoluble small chemicals (Bisphenol-A; BPA). Also, known problems of sol鈥揼el adhesion to conventional microtiter well plate substrates are circumvented by anchoring the sol鈥揼el microspots to a porous silion surface so-called, PS-SG chips. We confirmed low molecular weight chemical immobilization inside a sol鈥揼el network using fluorescein. BPA and the BPA specific aptamer were utilized as a model pair to verify the affinity specific interaction in the PS-SG selection system. The aptamer interacted specifically with BPA in the sol鈥揼el spots, as shown in microarrays forming the letters 鈥淟鈥? 鈥淯鈥? 鈥淣鈥? and 鈥淒鈥? Moreover, the bound aptamer was released by heat, recovered, and verified by gel electrophoresis. The developed PS-SG chip platform will be used for screening aptamers against numerous small molecules such as toxins, metabolites, or pesticide residues.