Reversed Phase Ion-Pair Liquid Chromatographic Determination of Nicotine in Commercial Tobacco Products. 1. Moist Snuff

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• 作者：Laura A. Ciolino ; Heather A. McCauley ; Diane B. Fraser ; Debra Y. Barnett ; Timothy Y. Yi ; and James A. Turner
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：1999
• 出版时间：September 1999
• 年：1999
• 卷：47
• 期：9
• 页码：3706 - 3712
• 全文大小：58K
• 年卷期：v.47,no.9(September 1999)
• ISSN：1520-5118

文摘

The official methods for the determination of nicotine in commercial tobacco products (AOAC,CORESTA) are based on approaches that are not selective for nicotine (colorimetric measurement,steam distillation, perchloric acid titration), and the availability of published methods based onstate-of-the-art chromatographic methods is limited. Reversed phase ion-pair liquid chromatographyhas been established as a viable alternative for the analysis of basic analytes. A reversed phaseion-pair liquid chromatographic method for the determination of nicotine in commercial tobaccoproducts was developed and optimized in separate experiments (Ciolino, L. A.; Turner, J. A.;McCauley, H. A.; Smallwood, A. W.; Yi, T. Y. J. Chromatogr. 1999a, 852 (2), 451-463). An extensivewithin-laboratory performance study of the optimized method was subsequently conducted, andresults are presented here for the determination of nicotine in commercial moist snuff. Results forthe determination of nicotine in commercial cigarettes are presented in a subsequent paper (Ciolino,L. A.; Fraser, D. B.; Yi, T. Y.; Turner, J. A.; Barnett, D. Y.; McCauley, H. A. J. Agric. Food Chem.1999b, 47, 3713-3717).Keywords: Nicotine; moist snuff; tobacco; alkaloids; reversed phase ion-pair liquid chromatography