Simultaneous Measurement of 19 Components in Serum-Containing Animal Cell Culture Media by Fourier Transform Near-Infrared Spectroscopy

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• 作者：Mark R. Riley ; Heather M. Crider ; Megan E. Nite ; Rafael A. Garcia ; Jennifer Woo ; and Rachel M. Wegge
• 刊名：Biotechnology Progress
• 出版年：2001
• 出版时间：April 2001
• 年：2001
• 卷：17
• 期：2
• 页码：376 - 378
• 全文大小：32K
• 年卷期：v.17,no.2(April 2001)
• ISSN：1520-6033

文摘

Animal cell cultures generate maximal amounts of desired products when maintainedin a controlled environment with low and constant concentrations of nutrients andwastes. Traditionally this has involved slow addition of glucose and glutamine;however, recent studies have indicated that a number of low concentration amino acidsare required to prevent initiation of apoptosis. Therefore, optimal control of animalcell cultures will likely require measurement of a large number of chemical components.We present here the evaluation of a near-infrared spectroscopic (NIRS) monitoringscheme to quantify 19 cellular nutrients and wastes in culture medium with andwithout serum. The components include glucose, lactate, ammonia, pyruvate, glutamine,and 14 other amino acids. Spectroscopic calibrations were generated for a syntheticversion of a standard culture medium (DMEM) in which the concentrations of 17DMEM components and ammonia and lactate were varied in a random fashion. Thisrandomization provides a stringent evaluation of the measurement scheme. Reasonablyaccurate measurements of these 19 components could be accomplished in the absenceor presence of 10% horse serum by volume with percent errors ranging from 3% to37%. Analytes with concentrations as low as 0.3 mM could be reliably quantified. Thepresence of serum, when properly included in the calibration, has little effect onmeasurement error. These results provide an important step toward application ofNIRS for monitoring the large number of varying components of animal cellcultivations.