In Cellulo Mapping of Subcellular Localized Bilirubin

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• 作者：Jong-Seok Park ; Eunju Nam ; Hye-Kyeong Lee ; Mi Hee Lim ; Hyun-Woo Rhee
• 刊名：ACS Chemical Biology
• 出版年：2016
• 出版时间：August 19, 2016
• 年：2016
• 卷：11
• 期：8
• 页码：2177-2185
• 全文大小：606K
• 年卷期：0
• ISSN：1554-8937

文摘

Bilirubin (BR) is a de novo synthesized metabolite of human cells. However, subcellular localization of BR in the different organelles of human cells has been largely unknown. Here, utilizing UnaG as a genetically encoded fluorescent BR sensor, we report the existence of relatively BR-enriched and BR-depleted microspaces in various cellular organelles of live cells. Our studies indicate that (i) the cytoplasmic facing membrane of the endoplasmic reticulum (ER) and the nucleus are relatively BR-enriched spaces and (ii) mitochondrial intermembrane space and the ER lumen are relatively BR-depleted spaces. Thus, we demonstrate a relationship between such asymmetrical BR distribution in the ER membrane and the BR metabolic pathway. Furthermore, our results suggest plausible BR-transport and BR-regulating machineries in other cellular compartments, including the nucleus and mitochondria.