Large Area Two-Dimensional B Cell Arrays for Sensing and Cell-Sorting Applications

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• 作者：Heejae Kim ; Junsang Doh ; Darrell J. Irvine ; Robert E. Cohen ; and Paula T. Hammond
• 刊名：Biomacromolecules
• 出版年：2004
• 出版时间：May 2004
• 年：2004
• 卷：5
• 期：3
• 页码：822 - 827
• 全文大小：962K
• 年卷期：v.5,no.3(May 2004)
• ISSN：1526-4602

文摘

Regular arrays of nonadherent B cells over large areas were produced with the use of micropatterned moleculartemplates consisting of a newly designed poly(allylamine)-g-poly(ethylene glycol) polycation graft copolymer.Polymer-on-polymer stamping (POPS) techniques were applied successfully to create micron scale patternsof the graft copolymer on negatively charged multilayer surfaces without losing resistance to the nonspecificadsorption of proteins. To generate templates for B cell arrays, the characteristics of the patterned surfacewere modified via introduction of surface biotinylation and specific protein adsorption. The qualities of Bcell arrays resulting from each template suggest the binding strength between nonadherent B cells and thetemplate surface is the controlling factor in the fabrication of clean and regular arrays of immobilizedlymphocytes over large areas, which is critical in many bio-technological and immunological applications.