Phosphonic Acid Monolayers for Binding of Bioactive Molecules to Titanium Surfaces

详细信息    查看全文

• 作者：Nina Adden ; Lara J. Gamble ; David G. Castner ; Andrea Hoffmann ; Gerhard Gross ; Henning Menzel
• 刊名：Langmuir
• 出版年：2006
• 出版时间：September 12, 2006
• 年：2006
• 卷：22
• 期：19
• 页码：8197 - 8204
• 全文大小：154K
• 年卷期：v.22,no.19(September 12, 2006)
• ISSN：1520-5827

文摘

Two different phosphonic acid monolayer films for immobilization of bioactive molecules such as the proteinBMP-2 on titanium surfaces have been prepared. Monolayers of (11-hydroxyundecyl)phosphonic acid and (12-carboxydodecyl)phosphonic acid molecules were produced by a simple dipping process (the T-BAG method). Theterminal functional groups on these monolayers were activated (carbonyldiimidazole for hydroxyl groups andN-hydroxysuccinimide for carboxyl groups) to bind amine-containing molecules. The reactivity of the surfaces wasinvestigated using trifluoroethylamine hydrochloride and BMP-2. Each step of the surface modification procedure wascharacterized by X-ray photoelectron spectroscopy and time-of-flight secondary ion mass spectrometry.