Resident Neuroelectrochemical Interfacing Using Carbon Nanofiber Arrays

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• 作者：Timothy E. McKnight ; Anatoli V. Melechko ; Benjamin L. Fletcher ; Stephen W. Jones ; Dale K. Hensley ; Diana B. Peckys ; Guy D. Griffin ; Michael L. Simpson ; M. Nance Ericson
• 刊名：Journal of Physical Chemistry B
• 出版年：2006
• 出版时间：August 10, 2006
• 年：2006
• 卷：110
• 期：31
• 页码：15317 - 15327
• 全文大小：1019K
• 年卷期：v.110,no.31(August 10, 2006)
• ISSN：1520-5207

文摘

Carbon nanofiber electrode architectures are used to provide for long-term, neuroelectroanalytical measurementsof the dynamic processes of intercellular communication between excitable cells. Individually addressed,vertically aligned carbon nanofibers are incorporated into multielement electrode arrays upon which excitablecell matrixes of both neuronal-like derived cell lines (rat pheochromocytoma, PC-12) and primary cells(dissociated cells from embryonic rat hippocampus) are cultured over extended periods (days to weeks).Electrode arrays are characterized with respect to their response to easily oxidized neurotransmitters, includingdopamine, norepinephrine, and 5-hydroxytyramide. Electroanalysis at discrete electrodes following long-term cell culture demonstrates that this platform remains responsive for the detection of easily oxidized speciesgenerated by the cultured cells. Preliminary data also suggests that quantal release of easily oxidized transmitterscan be observed at nanofiber electrodes following direct culture and differentiation on the arrays for periodsof at least 16 days.