Selective and Sensitive Detection of Heavy Metal Ions in 100% Aqueous Solution and Cells with a Fluorescence Chemosensor Based on Peptide Using Aggregation-Induced Emission

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• 作者：Lok Nath Neupane ; Eun-Taex Oh ; Heon Joo Park ; Keun-Hyeung Lee
• 刊名：Analytical Chemistry
• 出版年：2016
• 出版时间：March 15, 2016
• 年：2016
• 卷：88
• 期：6
• 页码：3333-3340
• 全文大小：540K
• ISSN：1520-6882

文摘

A fluorescent peptidyl chemosensor for the detection of heavy metal ions in aqueous solution as well as in cells was synthesized on the basis of the peptide receptor for the metal ions using an aggregation-induced emission fluorophore. The peptidyl chemosensor (1) bearing tetraphenylethylene fluorophore showed an exclusively selective turn-on response to Hg²⁺ among 16 metal ions in aqueous buffered solution containing NaCl. The peptidyl chemosensor complexed Hg²⁺ ions and then aggregated in aqueous buffered solution, resulting in the significant enhancement (OFF-On) of emissions at around 470 nm. The fluorescent sensor showed a highly sensitive response to Hg²⁺, and about 1.0 equiv of Hg²⁺ was enough for the saturation of the emission intensity change. The detection limit (5.3 nM, R² = 0.99) of 1 for Hg²⁺ ions was lower than the maximum allowable level of Hg²⁺ in drinking water by EPA. Moreover, the peptidyl chemosensor penetrated live cells and detected intracellular Hg²⁺ ions by the turn-on response.