Effect of Caseinophosphopeptides from 伪s- and 尾-Casein on Iron Bioavailability in HuH7 Cells

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• 作者：Mar铆a Jos茅 Garc铆a-Nebot ; Amparo Alegr铆a ; Reyes Barber谩 ; Fran莽ois Gaboriau ; Sa茂d Bouhallab
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2015
• 出版时间：August 5, 2015
• 年：2015
• 卷：63
• 期：30
• 页码：6757-6763
• 全文大小：360K
• ISSN：1520-5118

文摘

Two pools of caseinophosphopeptides (CPPs) obtained from 伪_s- and 尾-casein fractions (伪-CPPs and 尾-CPPs) were characterized. A total of 16 CPPs were identified in the 伪-CPPs pool, 9 of them derived from 伪_s1-casein and 7 from 伪_s2-casein. A total of 18 CPPs were identified in the 尾-CPPs pool. Four of the identified CPPs contained the characteristic phosphoseryl-glutamic acid cluster SpSpSpEE. Calcein assay was used to compare the iron-binding capacity of the 伪- and 尾-CPPs pools. At the concentration of 12.5 渭M CPPs used in the iron bioavailability assays, 尾-CPPs pools show greater iron-binding capacity than 伪-CPPs pools. HuH7 human hepatoma cells show many differentiated functions of liver cells in vivo and can be used to evaluate iron bioavailability (ferritin content and soluble transferrin receptor) from Fe鈭捨?CPPs and Fe鈭捨?CPPs complexes. The 伪-CPPs and 尾-CPPs pools did not improve ferritin content or soluble transferrin receptor in HuH7 cells.