Stable Isotope Labeling of Entire Bacillus atrophaeus Spores and Vegetative Cells Using Bioaerosol Mass Spectrometry

详细信息    查看全文

• 作者：Gregg A.Czerwieniec ; Scott C. Russell ; Herbert J. Tobias ; Maurice E. Pitesky ; David P. Fergenson ; Paul Steele ; Abneesh Srivastava ; Joanne M. Horn ; Matthias Frank ; Eric E. Gard ; and Carlito B. Lebrilla
• 刊名：Analytical Chemistry
• 出版年：2005
• 出版时间：February 15, 2005
• 年：2005
• 卷：77
• 期：4
• 页码：1081 - 1087
• 全文大小：207K
• 年卷期：v.77,no.4(February 15, 2005)
• ISSN：1520-6882

文摘

Single vegetative cells and spores of Bacillus atrophaeus, formerly Bacillus subtilis var. niger, wereanalyzed using bioaerosol mass spectrometry. Key biomarkers were identified from organisms grown in ¹³C and¹⁵N isotopically enriched media. Spore spectra containpeaks from dicipolinate and amino acids. The resultsindicate that compounds observed in the spectra correspond to material from the spore's core and not theexosporium. Standard compounds and mixtures wereanalyzed for comparison. The biomarkers for vegetativecells were clearly different from those of the spores,consisting mainly of phosphate clusters and amino acidfragments.