Mapping Mechanical Force Propagation through Biomolecular Complexes
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- 作者：Constantin Schoeler ; Rafael C. Bernardi ; Klara H. Malinowska ; Ellis Durner ; Wolfgang Ott ; Edward A. Bayer ; Klaus Schulten ; Michael A. Nash ; Hermann E. Gaub
- 刊名：Nano Letters
- 出版年：2015
- 出版时间：November 11, 2015
- 年：2015
- 卷：15
- 期：11
- 页码：7370-7376
- 全文大小：502K
- ISSN：1530-6992
文摘
Here we employ single-molecule force spectroscopy with an atomic force microscope (AFM) and steered molecular dynamics (SMD) simulations to reveal force propagation pathways through a mechanically ultrastable multidomain cellulosome protein complex. We demonstrate a new combination of network-based correlation analysis supported by AFM directional pulling experiments, which allowed us to visualize stiff paths through the protein complex along which force is transmitted. The results implicate specific force-propagation routes nonparallel to the pulling axis that are advantageous for achieving high dissociation forces.