Walnut Polyphenols Prevent Liver Damage Induced by Carbon Tetrachloride and D-Galactosamine: Hepatoprotective Hydrolyzable Tannins in the Kernel Pellicles of Walnut

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• 作者：Hiroshi Shimoda ; Junji Tanaka ; Mitsunori Kikuchi ; Toshiyuji Fukuda ; Hideyuki Ito ; Tsutomu Hatano ; Takashi Yoshida
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2008
• 出版时间：June 25, 2008
• 年：2008
• 卷：56
• 期：12
• 页码：4444 - 4449
• 全文大小：366K
• 年卷期：v.56,no.12(June 25, 2008)
• ISSN：1520-5118

文摘

The polyphenol-rich fraction (WP, 45% polyphenol) prepared from the kernel pellicles of walnuts was assessed for its hepatoprotective effect in mice. A single oral administration of WP (200 mg/kg) significantly suppressed serum glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) elevation in liver injury induced by carbon tetrachloride (CCl₄), while it did not suppress D-galactosamine (GalN)-induced liver injury. In order to identify the active principles in WP, we examined individual constituents for the protective effect on cell damage induced by CCl₄ and D-GalN in primary cultured rat hepatocytes. WP was effective against both CCl₄- and D-GalN-induced hepatocyte damages. Among the constituents, only ellagitannins with a galloylated glucopyranose core, such as tellimagrandins I, II, and rugosin C, suppressed CCl₄-induced hepatocyte damage significantly. Most of the ellagitannins including tellimagrandin I and 2,3-O-hexahydroxydiphenoylglucose exhibited remarkable inhibitory effect against D-GalN-induced damage. Telliamgrandin I especially completely suppressed both CCl₄- and D-GalN-induced cell damage, and thus is likely the principal constituent for the hepatoprotective effect of WP.