Longitudinal On-Column Thermal Modulation for Comprehensive Two-Dimensional Liquid Chromatography
详细信息 查看全文
- 作者：Mari E. Creese ; Mathew J. Creese ; Joe P. Foley ; Hernan J. Cortes ; Emily F. Hilder ; Robert A. Shellie ; Michael C. Breadmore
- 刊名：Analytical Chemistry
- 出版年：2017
- 出版时间：January 17, 2017
- 年：2017
- 卷：89
- 期：2
- 页码：1123-1130
- 全文大小：456K
- ISSN：1520-6882
文摘
Longitudinal on-column thermal modulation for comprehensive two-dimensional liquid chromatography is introduced. Modulation optimization involved a systematic investigation of heat transfer, analyte retention, and migration velocity at a range of temperatures. Longitudinal on-column thermal modulation was realized using a set of alkylphenones and compared to a conventional valve-modulator employing sample loops. The thermal modulator showed a reduced modulation-induced pressure impact than valve modulation, resulting in reduced baseline perturbation by a factor of 6; yielding a 6–14-fold improvement in signal-to-noise. A red wine sample was analyzed to demonstrate the potential of the longitudinal on-column thermal modulator for separation of a complex sample. Discrete peaks in the second dimension using the thermal modulator were 30–55% narrower than with the valve modulator. The results shown herein demonstrate the benefits of an active focusing modulator, such as reduced detection limits and increased total peak capacity.