The study of N-
linked g
lycosy
lation has
long been comp
licated by a
lack of bioinformatics too
ls. In particu
lar, there is sti
ll a
lack of fast and robust data processing too
ls for targeted (re
lative) quantitation. We have deve
loped modu
lar, high-throughput data processing software, MassyToo
ls, that is capab
le of ca
librating spectra, extracting data, and performing qua
lity contro
l ca
lcu
lations based on a user-defined
list of g
lycan or g
lycopeptide compositions. Typica
l examp
les of output inc
lude re
lative areas after background subtraction, isotopic pattern-based qua
lity scores, spectra
l qua
lity scores, and signa
l-to-noise ratios. We demonstrated MassyToo
ls鈥?performance on MALDI-TOF-MS g
lycan and g
lycopeptide data from different samp
les. MassyToo
ls yie
lded better ca
libration than the commercia
l software f
lexAna
lysis, genera
lly showing 2-fo
ld better ppm errors after interna
l ca
libration. Re
lative quantitation using MassyToo
ls and f
lexAna
lysis gave simi
lar resu
lts, yie
lding a re
lative standard deviation (RSD) of the main g
lycan of 鈭?%. However, MassyToo
ls yie
lded 2- to 5-fo
ld
lower RSD va
lues for
low-abundant ana
lytes than f
lexAna
lysis. Additiona
lly, feature curation based on the computed qua
lity criteria improved the data qua
lity. In conc
lusion, we show that MassyToo
ls is a robust automated data processing too
l for high-throughput, high-performance g
lycosy
lation ana
lysis. The package is re
leased under the Apache 2.0
license and is free
ly avai
lab
le on GitHub (
ls" class="extLink">https://github.com/Tarskin/MassyTools).