Analysis of the Fungicide Boscalid in Horticultural Crops Using an Enzyme-Linked Immunosorbent Assay and an Immunosensor Based on Surface Plasmon Resonance
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- 作者：Yuki Hirakawa ; Tomomi Yamasaki ; Ayako Harada ; Toshiya Ohtake ; Kayo Adachi ; Seiji Iwasa ; Hiroshi Narita ; Shiro Miyake
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2015
- 出版时间：September 16, 2015
- 年：2015
- 卷：63
- 期：36
- 页码：8075-8082
- 全文大小：339K
- ISSN：1520-5118
文摘
A direct competitive enzyme-linked immunosorbent assay (dc-ELISA) and an immunosensor based on surface plasmon resonance (SPR-sensor) were developed for fungicide boscalid determination in horticultural crops. To produce antiboscalid monoclonal antibodies (MoAb BSC7 and MoAb BSC72) for these assays, a hapten of boscalid was synthesized and conjugated to keyhole limpet hemocyanin for Balb/c mouse immunization. The working range of the dc-ELISA was 0.8鈥?6 ng/mL with MoAb BSC7 and 2.5鈥?20 ng/mL with MoAb BSC72, and that of the SPR-sensor was 17鈥?0 ng/mL with MoAb BSC7. The dc-ELISA and SPR-sensor were compared for their sensitivity in determining boscalid residues at the maximum residue limit of 1鈥?0 mg/kg for horticultural crops in Japan. Recovery of the spiked boscalid was 85鈥?09% by the SPR-sensor and 100鈥?24% by the dc-ELISA. On real tomato samples, the results obtained by both of these immunoassays correlated well with the results obtained by high-performance liquid chromatography.