Quantum Dot FRET Biosensors that Respond to pH, to Proteolytic or Nucleolytic Cleavage, to DNA Synthesis, or to a Multiplexing Combination

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• 作者：Miho Suzuki ; Yuzuru Husimi ; Hirokazu Komatsu ; Koji Suzuki ; Kenneth T. Douglas
• 刊名：Journal of the American Chemical Society
• 出版年：2008
• 出版时间：April 30, 2008
• 年：2008
• 卷：130
• 期：17
• 页码：5720 - 5725
• 全文大小：523K
• 年卷期：v.130,no.17(April 30, 2008)
• ISSN：1520-5126

文摘

Fluorescent acceptors have been immobilized on nanoparticulate quantum dots (QDs), which serve in turn as their FRET donors. The broad excitation and narrow emission bands of QDs mark them as having excellent potential as donors for FRET and, in principle, differently colored QDs could be excited simultaneously. The present work describes the preparation and operation of FRET-based QD bioprobes individually able to detect the actions of protease, deoxyribonuclease, DNA polymerase, or changes in pH. In addition, two such QD-mounted biosensors were excited at a single wavelength, and shown to operate simultaneously and independently of each other in the same sample solution, allowing multiplex detection of the action of a protease, trypsin, in the presence of deoxyribonuclease.