We have observed and analyzed a universal temperature crossover behavior of electrical conductance in a single oligothiophene molecular wire. The crossover between the Arrhenius-type temperature dependence at high temperature and the temperature-invariant behavior at low temperature is found at a critical molecular wire length of 5.6 nm, where we found a change from the exponential length dependence to the length-invariant behavior. We have derived a scaling function analysis for the origin of the crossover behavior. After assuring that the analysis fits the explanation of the Keldysh Green鈥檚 function calculation for the temperature dependence, we have applied it to our experimental results and found successfully that our scaling function gives a universal description of the temperature dependence for all over the temperature range.