Adsorption Behavior of Microbes on a QCM Chip Modified with an Artificial Siderophore鈥揊e3+ Complex

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• 作者：Tomohiko Inomata ; Hiroshi Eguchi ; Yasuhiro Funahashi ; Tomohiro Ozawa ; Hideki Masuda
• 刊名：Langmuir
• 出版年：2012
• 出版时间：January 17, 2012
• 年：2012
• 卷：28
• 期：2
• 页码：1611-1617
• 全文大小：400K
• 年卷期：v.28,no.2(January 17, 2012)
• ISSN：1520-5827

文摘

Three hydroxamate-type artificial siderophores with terminal NH₂ groups, tris[2-{3-(N-acyl-N-hydroxamino)propylamido}propyl]aminomethane (1鈥?b>3, acyl-R group = Me, Et, and Ph, respectively), and their Fe³⁺ complexes, 4鈥?b>6, were prepared. The stability constant (log 尾) of 4 was estimated to be about 31 by its EDTA titration. The biological activities of 4鈥?b>6 for Microbacterium flavescens, which is a hydroxamate-type siderophore, auxotrophic gram-positive microbe, clearly indicated that they permeated the cell membrane depending on their terminal bulky acyl-R groups. These artificial siderophore complexes, 4鈥?b>6, were modified on Au electrode surfaces with the terminal NH₂ group (4鈥?b>6/Au). The surface modification of 4鈥?b>6 was confirmed by several electrochemical measurements. The quartz crystal microbalance (QCM) chips were also modified with 4鈥?b>6. Microbe adsorption measurements using these modified QCM chips for M. flavescens, Pseudomonas putida, and Eschrichia coli were performed. The QCM chips have the ability to adsorb microbes selectively as a result of the differences in the interactions between the structures of Fe³⁺鈥揳rtificial siderophore complexes and their receptors or binding proteins within the cell membrane.