Immobilized Diaphorase Surfaces Observed by Scanning Electrochemical Microscope with Shear Force Based Tip-Substrate Positioning
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- 作者：Hiroshi Yamada ; Hikaru Fukumoto ; Tetsuya Yokoyama ; and Tohru Koike
- 刊名：Analytical Chemistry
- 出版年：2005
- 出版时间：March 15, 2005
- 年：2005
- 卷：77
- 期：6
- 页码：1785 - 1790
- 全文大小：338K
- 年卷期：v.77,no.6(March 15, 2005)
- ISSN：1520-6882
文摘
Imaging of a coimmobilized diaphorase and albuminsurface was investigated by scanning electrochemicalmicroscopy (SECM) with shear force based tip-substratedistance control. A microelectrode tip was attached to acommercially available tuning fork to detect the shearforce between the microelectrode tip and the surface. Weused the standing approach mode, which repeats anapproach and retraction at each data point of the surfaceto obtain simultaneous current and topographic images.To check the performance of our SECM system, weimaged a platinum-patterned array electrode and a diaphorase/albumin coimmobilized glass surface. Since thesystem acquires current when the tip is retracted to adesired distance, this mode is useful for a relatively largemicroelectrode (~10 
m) and for scanning a large area(few hundreds of micrometers). Furthermore, by retracting the tip when the tip moves laterally to the next datapoint to avoid contact between the tip and sample surface,we successfully imaged the surface without destroying itsmorphology.
m) and for scanning a large area(few hundreds of micrometers). Furthermore, by retracting the tip when the tip moves laterally to the next datapoint to avoid contact between the tip and sample surface,we successfully imaged the surface without destroying itsmorphology.