Accelerated Polymer鈥揚olymer Click Conjugation by Freeze鈥揟haw Treatment

详细信息    查看全文

• 作者：Hiroyasu Takemoto ; Kanjiro Miyata ; Takehiko Ishii ; Shota Hattori ; Shigehito Osawa ; Nobuhiro Nishiyama ; Kazunori Kataoka
• 刊名：Bioconjugate Chemistry
• 出版年：2012
• 出版时间：August 15, 2012
• 年：2012
• 卷：23
• 期：8
• 页码：1503-1506
• 全文大小：240K
• 年卷期：v.23,no.8(August 15, 2012)
• ISSN：1520-4812

文摘

Herein, we report a unique technique to accelerate polymer鈥揝NA conjugation based on copper-free click chemistry: gradual freeze鈥搕hawing of the reaction solution substantially increases the conjugation rate possibly because of the reactant concentration at the microenvironment scale. This technique was applied to the conjugation between a small interfering RNA (siRNA) and PEG in an aqueous buffer at/below room temperature.