Identification, Characterization, and Three-Dimensional Structure of the Novel Circular Bacteriocin, Enterocin NKR-5-3B, from Enterococcus faecium
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- 作者：Kohei Himeno ; K. Johan Rosengren ; Tomoko Inoue ; Rodney H. Perez ; Michelle L. Colgrave ; Han Siean Lee ; Lai Y. Chan ; S贸nia Troeira Henriques ; Koji Fujita ; Naoki Ishibashi ; Takeshi Zendo ; Pongtep Wilaipun ; Jiro Nakayama ; Vichien Leelawatcharamas ; Hiroyuki Jikuya ; David J. Craik ; Kenji Sonomoto
- 刊名：Biochemistry
- 出版年：2015
- 出版时间：August 11, 2015
- 年：2015
- 卷：54
- 期：31
- 页码：4863-4876
- 全文大小：687K
- ISSN：1520-4995
文摘
Enterocin NKR-5-3B, one of the multiple bacteriocins produced by Enterococcus faecium NKR-5-3, is a 64-amino acid novel circular bacteriocin that displays broad-spectrum antimicrobial activity. Here we report the identification, characterization, and three-dimensional nuclear magnetic resonance solution structure determination of enterocin NKR-5-3B. Enterocin NKR-5-3B is characterized by four helical segments that enclose a compact hydrophobic core, which together with its circular backbone impart high stability and structural integrity. We also report the corresponding structural gene, enkB, that encodes an 87-amino acid precursor peptide that undergoes a yet to be described enzymatic processing that involves adjacent cleavage and ligation of Leu24 and Trp87 to yield the mature (circular) enterocin NKR-5-3B.