Gangliosides and Ceramides Change in a Mouse Model of Blast Induced Traumatic Brain Injury

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• 作者：Amina S. Woods ; Benoit Colsch ; Shelley N. Jackson ; Jeremy Post ; Kathrine Baldwin ; Aurelie Roux ; Barry Hoffer ; Brian M. Cox ; Michael Hoffer ; Vardit Rubovitch ; Chaim G. Pick ; J. Albert Schultz ; Carey Balaban
• 刊名：ACS Chemical Neuroscience
• 出版年：2013
• 出版时间：April 17, 2013
• 年：2013
• 卷：4
• 期：4
• 页码：594-600
• 全文大小：488K
• 年卷期：v.4,no.4(April 17, 2013)
• ISSN：1948-7193

文摘

Explosive detonations generate atmospheric pressure changes that produce nonpenetrating blast induced 鈥渕ild鈥?traumatic brain injury (bTBI). The structural basis for mild bTBI has been extremely controversial. The present study applies matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging to track the distribution of gangliosides in mouse brain tissue that were exposed to very low level of explosive detonations (2.5鈥?.5 psi peak overpressure). We observed major increases of the ganglioside GM2 in the hippocampus, thalamus, and hypothalamus after a single blast exposure. Moreover, these changes were accompanied by depletion of ceramides. No neurological or brain structural signs of injury could be inferred using standard light microscopic techniques. The first source of variability is generated by the Latency between blast and tissue sampling (peak intensity of the blast wave). These findings suggest that subtle molecular changes in intracellular membranes and plasmalemma compartments may be biomarkers for biological responses to mild bTBI. This is also the first report of a GM2 increase in the brains of mature mice from a nongenetic etiology.