Surface Plasmon Resonance Sensor Based on Magnetic Molecularly Imprinted Polymers Amplification for Pesticide Recognition

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• 作者：Gui-Hong Yao ; Ru-Ping Liang ; Chun-Fang Huang ; Ying Wang ; Jian-Ding Qiu
• 刊名：Analytical Chemistry
• 出版年：2013
• 出版时间：December 17, 2013
• 年：2013
• 卷：85
• 期：24
• 页码：11944-11951
• 全文大小：422K
• 年卷期：v.85,no.24(December 17, 2013)
• ISSN：1520-6882

文摘

We reported here a method to enhance detection sensitivity in surface plasmon resonance (SPR) spectroscopy integrated with a surface molecular imprinting recognition system and employing magnetic molecular imprinting polymer nanoparticles for amplifying SPR response. The proposed magnetic molecular imprinting polymer was designed by self-polymerization of dopamine on the Fe₃O₄ NPs surface in weak base aqueous solution in the presence of template chlorpyrifos (CPF). The imprinted Fe₃O₄@polydopamine nanoparticles (Fe₃O₄@PDA NPs) were characterized by Fourier transform infrared spectroscopy, UV鈥搗is absorption spectroscopy, and transmission electron microscopy. The biosensor showed a good linear relationship between the SPR angle shift and the chlorpyrifos concentration over a range from 0.001 to 10 渭M with a detection limit of 0.76 nM. A significant increase in sensitivity was therefore afforded through the use of imprinted Fe₃O₄@PDA NPs as an amplifier, and meanwhile, the imprinted Fe₃O₄@PDA NPs had an excellent recognition capacity to chlorpyrifos over other pesticides. The excellent sensitivity and selectivity and high stability of the designed biosensor make this magnetic imprinted Fe₃O₄@PDA NP an attractive recognition element for various SPR sensors for detecting pesticide residuals and other environmentally deleterious chemicals.