Yeast α-Glucosidase Inhibition by Isoflavones from Plants of Leguminosae as an in Vitro Alternative to Acarbose

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• 作者：Chun Whan Choi ; Yeon Hee Choi ; Mi-Ran Cha ; Dae Seok Yoo ; Young Sup Kim ; Gyu Hwan Yon ; Kyung Sik Hong ; Young Ho Kim ; Shi Yong Ryu
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2010
• 出版时间：September 22, 2010
• 年：2010
• 卷：58
• 期：18
• 页码：9988-9993
• 全文大小：829K
• 年卷期：v.58,no.18(September 22, 2010)
• ISSN：1520-5118

文摘

In the course of searching for new classes of α-glucosidase inhibitors originated from natural resources, 11 kinds of isoflavones, i.e., medicarpin (1), formononetin (2), mucronulatol (3), (3R)-calussequinone (5), (3R)-5′-methoxyvestitol (6), tectorigenin (7), biochanin A (8), tuberosin (9), calycosin (10), daidzein (11), and genistein (12), as well as a flavone, liquritigenin (4), were isolated as active principles responsible for the yeast α-glucosidase inhibitory activity from two leguminous plant extracts, i.e., the heartwood extract of Dalbergia odorifera and the roots extract of Pueraria thunbergiana. Each components (1−12) demonstrated a significantly potent inhibition on yeast α-glucosidase in a dose dependent manner when the p-nitrophenyl-α-d-glucopyranoside was used as a substrate in vitro. The concentration required for 50% enzyme inhibition (IC₅₀) were calculated as 2.93 mM (1), 0.51 mM (2), 3.52 mM (7) 0.35 mM (8), 3.52 mM (9), 0.85 mM (11), and 0.15 mM (12) when that of reference drug acarbose was evaluated as 9.11 mM, in vitro. However, isoflavone glycosides, i.e., puerarin (13), daidzin (14), formononetin-7-O-β-glucopyranoside (15), and genistin (16), exhibited a relatively poor inhibitory activity on yeast α-glucosidase as compared with the corresponding isoflavone (2, 11, 12), respectively

Keywords:

Dalbergia odorifera; Pueraria thunbergiana; Leguminosae; yeast α-glucosidase; isoflavone