Lipase Immobilization on Hyper-Cross-Linked Polymer-Coated Silica for Biocatalytic Synthesis of Phytosterol Esters with Controllable Fatty Acid Composition

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• 作者：Ming-Ming Zheng ; Yong Lu ; Feng-Hong Huang ; Lian Wang ; Ping-Mei Guo ; Yu-Qi Feng ; Qian-Chun Deng
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2013
• 出版时间：January 9, 2013
• 年：2013
• 卷：61
• 期：1
• 页码：231-237
• 全文大小：362K
• 年卷期：v.61,no.1(January 9, 2013)
• ISSN：1520-5118

文摘

In this study, a novel mixed-mode composite material, SiO₂@P(MAA-co-VBC-co-DVB), was prepared via the hyper-cross-linking of its precursor, which was produced via suspension polymerization in the presence of SiO₂ particles. Candida rugosa lipase (CRL) was immobilized on the SiO₂@P(MAA-co-VBC-co-DVB) particles via hydrophobic and weak cation-exchange interaction. The resulting immobilized CRL showed much better thermal stability and reusability in comparison to free CRL. On the basis of the excellent biocatalyst prepared, a method for high-efficiency enzymatic esterification of phytosterols with different fatty acids to produce the corresponding phytosterol esters was developed. Six phytosterol esters with conversions above 92.1% and controllable fatty acid composition were obtained under the optimized conditions: 80 渭mol/mL phytosterols, 160 渭mol/mL linolenic acid, and 15 mg/mL CRL@HPCS at 300 rpm and 50 掳C for 7 h in 30 mL of isooctane. The prepared phytosterol esters possessed a low acid value (鈮?.86 mg of KOH/g), peroxide value (鈮?.3 mequiv/kg), and conjugated diene value (鈮?.74 mmol/kg) and high purity (鈮?7.8%) and fatty solubility (鈮?8.9 g/100 mL). All the characteristics favored the wide application of phytosterol esters with controllable fatty acid composition in different fields of functional food.

Keywords:

phytosterol ester; biocatalysis; esterification; hyper-cross-linked polymer; fatty acid composition