Generalized Platform for Antibody Detection using the Antibody Catalyzed Water Oxidation Pathway
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- 作者：M. Elizabeth Welch ; Nicole L. Ritzert ; Hongjun Chen ; Norah L. Smith ; Michele E. Tague ; Youyong Xu ; Barbara A. Baird ; Héctor D. Abru?a ; Christopher K. Ober
- 刊名：Journal of the American Chemical Society
- 出版年：2014
- 出版时间：February 5, 2014
- 年：2014
- 卷：136
- 期：5
- 页码：1879-1883
- 全文大小：285K
- ISSN：1520-5126
文摘
Infectious diseases, such as influenza, present a prominent global problem including the constant threat of pandemics that initiate in avian or other species and then pass to humans. We report a new sensor that can be specifically functionalized to detect antibodies associated with a wide range of infectious diseases in multiple species. This biosensor is based on electrochemical detection of hydrogen peroxide generated through the intrinsic catalytic activity of all antibodies: the antibody catalyzed water oxidation pathway (ACWOP). Our platform includes a polymer brush-modified surface where specific antibodies bind to conjugated haptens with high affinity and specificity. Hydrogen peroxide provides an electrochemical signal that is mediated by Resorufin/Amplex Red. We characterize the biosensor platform, using model anti-DNP antibodies, with the ultimate goal of designing a versatile device that is inexpensive, portable, reliable, and fast. We demonstrate detection of antibodies at concentrations that fall well within clinically relevant levels.