Gold Nanoparticles Modify the Photophysical and Photochemical Properties of 6-Thioguanine: Preventing DNA Oxidative Damage

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• 作者：Rennian Wang ; Ling Yue ; Youqing Yu ; Xiaoran Zou ; Di Song ; Kunhui Liu ; Yajun Liu ; Hongmei Su
• 刊名：Journal of Physical Chemistry C
• 出版年：2016
• 出版时间：July 7, 2016
• 年：2016
• 卷：120
• 期：26
• 页码：14410-14415
• 全文大小：412K
• 年卷期：0
• ISSN：1932-7455

文摘

For the widely used anticancer, antiinflammatory, and immunosuppressant drug 6-thioguanine (6-TG), its unique photochemistry of absorbing UVA, generating singlet oxygen (¹O₂), and inducing oxidative DNA damage can cause severe phototoxicity side effects in biomedical applications. Here we raise a new strategy of loading 6-TG onto gold nanoparticles (AuNPs) and demonstrate from systematic ultraviolet irradiation experiments that the 6-TG photochemistry can be successfully modulated by binding with AuNPs and the phototoxicity under UVA irradiation are effectively removed, not only for single base 6-TG but also for 6-TG embedded in DNA. In conjunction with excited state ab initio calculations, the molecular mechanisms are further unveiled, accounting for the protection effects of AuNPs in prohibiting the 6-TG photoactivation and ¹O₂ oxidation. These findings point to tremendous possibilities of using AuNPs as inert carrier to modify excited state photochemistry and prevent unwanted phototoxicity effects of the adsorbed drug molecules.