Wire-in-Tube IrOx Architectures: Alternative Label-Free Immunosensor for Amperometric Immunoassay toward 伪-Fetoprotein

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• 作者：Qingling Li ; Dali Liu ; Lin Xu ; Ruiqing Xing ; Wei Liu ; Kuang Sheng ; Hongwei Song
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2015
• 出版时间：October 14, 2015
• 年：2015
• 卷：7
• 期：40
• 页码：22719-22726
• 全文大小：442K
• ISSN：1944-8252

文摘

A sensitive, label-free immunosensor based on iridium oxide (IrO_x, 0 鈮?x 鈮?2) nanofibers, which were synthesized through a simple one-spinneret electrospinning method, was first developed for immunoassay of the cancer biomarker 伪-fetoprotein (AFP). The specific wire-in-tube nanostructure could be obtained and the composition of IrO_x nanofibers also could be controlled through changing the annealing temperature. The unique structure and properties of IrO_x nanofibers obtained at 500 掳C not only led to increased electrode surface area and accelerated electron transfer kinetics but also could provide a highly stable matrix for the convenient conjugation of biomolecules together with chitosan (CS). The good electrochemical properties of the IrO_x-nanofiber-modified immunosensor allowed one to detect AFP over a wide concentration range from 0.05 to 150 ng/mL, with a detection limit of 20 pg/mL. The proposed immunosensor also has been used to determine AFP in human serum with satisfactory results. The present protocol was shown to be quite promising for clinical screening of cancer biomarkers and point-of-care diagnostics applications.