Recognizing Uncertainty Increases Robustness and Reproducibility of Mass Spectrometry-based Protein Inferences
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- 作者：Oliver Serang ; Luminita Moruz ; Michael R. Hoopmann ; Lukas K盲ll
- 刊名：Journal of Proteome Research
- 出版年：2012
- 出版时间：December 7, 2012
- 年：2012
- 卷：11
- 期：12
- 页码：5586-5591
- 全文大小：203K
- 年卷期：v.11,no.12(December 7, 2012)
- ISSN：1535-3907
文摘
Parsimony and protein grouping are widely employed to enforce economy in the number of identified proteins, with the goal of increasing the quality and reliability of protein identifications; however, in a counterintuitive manner, parsimony and protein grouping may actually decrease the reproducibility and interpretability of protein identifications. We present a simple illustration demonstrating ways in which parsimony and protein grouping may lower the reproducibility or interpretability of results. We then provide an example of a data set where a probabilistic method increases the reproducibility and interpretability of identifications made on replicate analyses of Human Du145 prostate cancer cell lines.r>