Photoinduced Immobilization of Biomolecules on the Surface of Azopolymer Films and Its Dependence on the Concentration and Type of the Azobenzene Moiety
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- 作者：Mamiko Narita ; Fumihiko Hoshino ; Makoto Mouri ; Masaaki Tsuchimori ; Taiji Ikawa ; Osamu Watanabe
- 刊名：Macromolecules
- 出版年：2007
- 出版时间：February 6, 2007
- 年：2007
- 卷：40
- 期：3
- 页码：623 - 629
- 全文大小：279K
- 年卷期：v.40,no.3(February 6, 2007)
- ISSN：1520-5835
文摘
We report on the photoinduced immobilization of Immunoglobulin G (IgG) on the surface ofazobenzene-bearing acrylate copolymers (azopolymers). Two different types of azopolymers were synthesizedthat incorporated either a 4-aminoazobenzene moiety (H-azopolymer) or a 4-aminocyanoazobenzene moiety (CN-azopolymer), using different concentrations of the respective moieties. IgG was immobilized on the surfaces ofthe azopolymer films by exposure to visible light, and each of the films was then treated with an aqueous solutionof an antigen. Antigen-antibody reactions were confirmed on the surfaces of the films, indicating that immobilizedIgG generated by a photoirradiation can retain its activity. The amount of the immobilized antibodies on theazopolymer surfaces increased with azobenzene content up to 30 wt % and saturated over 30 wt % when measuredunder the same conditions. The efficiency of the immobilization process was found to correlate with the depthof deformations on the surfaces of the azopolymers, which were characterized by comparison with deformationsinduced by polystyrene microspheres under the same conditions. The increased contact area produced byphotodeformation could enhance the interactions between the antibodies and the azopolymer, thereby causingthe antibodies to be more firmly immobilized. However, the photoimmobilization of IgG on H-azopolymers wassuperior to that on CN-azopolymers, even though their original hydrophilicities and adsorption efficiencies werealmost the same. We confirmed that both the photoisomerization processes and retention rates of the immobilizedantibodies were different for H- and CN-azopolymers. This suggests that the effectiveness of photoimmobilizationis controlled not only by photodeformation but also by retention capability, which in turn depends on the chemicalstructure after photoirradiation.