Neuroprotective Effects of Resveratrol on Cerebral Ischemia-Induced Neuron Loss Mediated by Free Radical Scavenging and Cerebral Blood Flow Elevation

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• 作者：Kwok Tung Lu ; Robin Y. Y. Chiou ; Li Ging Chen ; Ming Hsiang Chen ; Wan Ting Tseng ; Hsiang Tsang Hsieh ; and Yi Ling Yang
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2006
• 出版时间：April 19, 2006
• 年：2006
• 卷：54
• 期：8
• 页码：3126 - 3131
• 全文大小：226K
• 年卷期：v.54,no.8(April 19, 2006)
• ISSN：1520-5118

文摘

Resveratrol is a natural phytoestrogen and possesses many biological functions such as anti-inflammatory activity and protection against atherosclerosis and myocardial infraction. The presentstudy was carried out to elucidate the neuroprotective effect and possible mechanism of resveratrolon cerebral ischemia-induced hippocampus neuron loss. Sixty adult male rats underwent generalanesthesia (urethane, 1.4 g/kg, i.p.) and were divided into three groups: sham operation, ischemiatreatment, and ischemia combined with resveratrol administration (20 mg/kg, i.v.). The carotid arterywas bilaterally ligated to induce cerebral ischemia. Microdialysis and high-performance liquidchromatography were used to analyze dihydroxybenzoic acid (DHBA) that reflected the hippocampalhydroxyl radical level. Hippocampal nitric oxide was assayed among different groups. During cerebralischemia, the hydroxyl radical levels were elevated in rats and animals displayed severe neuronalloss. A single dose of resveratrol significantly increased the nitric oxide level and decreased thehydroxyl radical level. The reduction of cerebral blood flow and neuronal loss were also attenuatedby resveratrol treatment. The results demonstrated that a single infusion of resveratrol could elicitneuroprotective effects on cerebral ischemia-induced neuron damage through free radical scavengingand cerebral blood elevation due to NO release.Keywords: Phytoestrogen; ischemia; free radicals; nitric oxide; neuronal damage