Inhibition of TNF-伪-Induced Inflammation by Andrographolide via Down-Regulation of the PI3K/Akt Signaling Pathway

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• 作者：Haw-Wen Chen ; Ai-Hsuan Lin ; Hsing-Chin Chu ; Chien-Chun Li ; Chia-Wen Tsai ; Che-Yi Chao ; Chau-Jong Wang ; Chong-Kuei Lii ; Kai-Li Liu
• 刊名：Journal of Natural Products
• 出版年：2011
• 出版时间：November 28, 2011
• 年：2011
• 卷：74
• 期：11
• 页码：2408-2413
• 全文大小：283K
• 年卷期：v.74,no.11(November 28, 2011)
• ISSN：1520-6025

文摘

Andrographolide (1), an active constituent of Andrographis paniculata, decreased tumor necrosis factor-伪 (TNF-伪)-induced intercellular adhesion molecule-1 (ICAM-1) expression and adhesion of HL-60 cells onto human umbilical vein endothelial cells (HUVEC), which are associated with inflammatory diseases. Moreover, 1 abolished TNF-伪-induced Akt phosphorylation. Transfection of an activated Akt1 cDNA vector increased Akt phosphorylation and ICAM-1 expression like TNF-伪. In addition, 1 and LY294002 blocked TNF-伪-induced I魏B-伪 degradation and nuclear p65 protein accumulation, as well as the DNA-binding activity of NF-魏B. Compound 1 exhibits anti-inflammatory properties through the inhibition of TNF-伪-induced ICAM-1 expression. The anti-inflammatory activity of 1 may be associated with the inhibition of the PI3K/Akt pathway and downstream target NF-魏B activation in HUVEC cells.