Automated On-Line Sample Pretreatment System for the Determination of Trace Metals in Biological Samples by Inductively Coupled Plasma Mass Spectrometry
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- 作者：Chuan-Chieh Huang ; Mo-Hsiung Yang ; and Tung-Sheng Shih
- 刊名：Analytical Chemistry
- 出版年：1997
- 出版时间：October 1, 1997
- 年：1997
- 卷：69
- 期：19
- 页码：3930 - 3939
- 全文大小：173K
- 年卷期：v.69,no.19(October 1, 1997)
- ISSN：1520-6882
文摘
A fully automated on-line sample pretreatment systemcombining microwave digestion with sample preconcentration/matrix separation for the determination of tracemetals (Fe, Ni, Cu, Zn, and Pb) in blood and serumsamples by inductively coupled plasma mass spectrometry (ICPMS) was designed and evaluated. The sampleswere mixed with an appropriate reagent and digested ina flow-through, focused microwave-heated oven. Afterdigestion, the sample solution was transferred on-line toa column packed with iminodiacetate-based resin forseparation of matrix elements like Na, K, Ca, Mg, Cl, P,and S that might interfere with the measurement byICPMS. The analytes chelated on the resin were subsequently eluted and led to ICPMS for multielement determination. The whole sample pretreatment process wasautomatically controlled by a self-designed expert system.The analytical reliability of data from this on-linesystemwas confirmed to be good with the analysis of SRMsamples (Seronorm Whole Blood and NIST SRM 1598Bovine Serum), and the limits of detection (3
) for Fe,Ni, Cu, Zn, and Pb were 68, 0.34, 3.5, 13.4, and 0.22
g/L, respectively. With this fully automatedon-linesystem, the determination of analytes in biological fluidsamples down to micrograms-per-liter levels has beenproven to be feasible, and the sample throughput canachieve up to 6 samples/h.
) for Fe,Ni, Cu, Zn, and Pb were 68, 0.34, 3.5, 13.4, and 0.22g/L, respectively. With this fully automatedon-linesystem, the determination of analytes in biological fluidsamples down to micrograms-per-liter levels has beenproven to be feasible, and the sample throughput canachieve up to 6 samples/h.