A Cell-Based Screening Identifies Compounds from the Stem of Momordica charantia that Overcome Insulin Resistance and Activate AMP-Activated Protein Kinase
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- 作者：Hsueh-Ling Cheng ; Hsin-Kai Huang ; Chi-I Chang ; Chung-Pao Tsai ; Chang-Hung Chou
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2008
- 出版时间：August 27, 2008
- 年：2008
- 卷：56
- 期：16
- 页码：6835-6843
- 全文大小：523K
- 年卷期：v.56,no.16(August 27, 2008)
- ISSN：1520-5118
文摘
Treatment of insulin resistance is a critical strategy in the prevention and management of type 2 diabetes. The crude extracts from all parts of Momordica charantia L. have been reported by many studies for the effective treatment of diabetes and related complications. However, the exact ingredients responsible for the hypoglycemic effect and the underlying mechanism of their actions have not been well characterized because of the lack of a proper assay and screening system. A new cell-based, nonradioactive, and nonfluorescent screening method was demonstrated in this study to screen for natural products from the stem of M. charantia, aiming to identify hypoglycemic components that can overcome cellular insulin resistance. The results suggest triterpenoids being potential hypoglycemic components of the plant and the mechanism underlying their action involving AMP-activated protein kinase.