Designed Diblock Oligonucleotide for the Synthesis of Spatially Isolated and Highly Hybridizable Functionalization of DNA鈥揋old Nanoparticle Nanoconjugates

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• 作者：Hao Pei ; Fan Li ; Ying Wan ; Min Wei ; Huajie Liu ; Yan Su ; Nan Chen ; Qing Huang ; Chunhai Fan
• 刊名：The Journal of the American Chemical Society
• 出版年：2012
• 出版时间：July 25, 2012
• 年：2012
• 卷：134
• 期：29
• 页码：11876-11879
• 全文大小：252K
• 年卷期：v.134,no.29(July 25, 2012)
• ISSN：1520-5126

文摘

Conjugates of DNA and gold nanoparticles (AuNPs) typically exploit the strong Au鈥揝 chemistry to self-assemble thiolated oligonucleotides at AuNPs. However, it remains challenging to precisely control the orientation and conformation of surface-tethered oligonucleotides and finely tune the hybridization ability. We herein report a novel strategy for spatially controlled functionalization of AuNPs with designed diblock oligonucleotides that are free of modifications. We have demonstrated that poly adenine (polyA) can serve as an effective anchoring block for preferential binding with the AuNP surface, and the appended recognition block adopts an upright conformation that favors DNA hybridization. The lateral spacing and surface density of DNA on AuNPs can also be systematically modulated by adjusting the length of the polyA block. Significantly, this diblock oligonucleotide strategy results in DNA鈥揂uNPs nanoconjugates with high and tunable hybridization ability, which form the basis of a rapid plasmonic DNA sensor.