Orthogonal Spin Labeling and Gd(III)鈥揘itroxide Distance Measurements on Bacteriophage T4-Lysozyme
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- 作者：Luca Garbuio ; Enrica Bordignon ; Evan K. Brooks ; Wayne L. Hubbell ; Gunnar Jeschke ; Maxim Yulikov
- 刊名：The Journal of Physical Chemistry B
- 出版年：2013
- 出版时间：March 21, 2013
- 年：2013
- 卷：117
- 期：11
- 页码：3145-3153
- 全文大小：480K
- 年卷期：v.117,no.11(March 21, 2013)
- ISSN：1520-5207
文摘
We present the first example of chemoselective site-specific spin labeling of a monomeric protein with two spectroscopically orthogonal spin labels: a gadolinium(III) chelate complex and a nitroxide radical. A detailed analysis of the performance of two commercially available Gd(III) ligands in the Gd(III)鈥搉itroxide pulse double electron鈥揺lectron resonance (DEER or PELDOR) experiment is reported. A modification of the flip angle of the pump pulse in the Gd(III)鈥搉itroxide DEER experiment is proposed to optimize sensitivity.