Inhibitory Activities of Semicarbazide-Sensitive Amine Oxidase and Angiotensin Converting Enzyme of Pectin Hydroxamic Acid
详细信息 查看全文
- 作者：Wen-Chi Hou ; Mei-Hsien Lee ; Feng-Lin Hsu ; and Yaw-Huei Lin
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2003
- 出版时间：October 8, 2003
- 年：2003
- 卷：51
- 期：21
- 页码：6362 - 6366
- 全文大小：99K
- 年卷期：v.51,no.21(October 8, 2003)
- ISSN：1520-5118
文摘
Solutions of 100 mL of 1% commercial pectin each with a different degree of esterification (DE),DE94, DE65, and DE25, were reacted with 100 mL of 2 M alkaline hydroxylamine (pH 12.0) at roomtemperature for 4 or 18 h. These pectin hydroxamic acids (PHAs; DE94T4, DE94T18, DE65T4, andDE25T4) were used to test the inhibitory activities against semicarbazide-sensitive amine oxidase(SSAO) and angiotensin-converting enzyme (ACE). Compared to different DE pectins (DE94, DE65,and DE25), the PHAs of DE94T4, DE94T18, DE65T4, and DE25T4 showed different inhibition activitiesagainst SSAO or ACE. Commercial pectins with different DE values showed negligible SSAO orACE inhibitions. The order of SSAO inhibition was DE65T4 > DE94T18 
DE25T4 
DE94T4.However, the order of ACE inhibition was DE94T4 > DE94T18 DE65T4 > DE25T4. The SSAOactivity staining or ACE-hydrolyzed products on TLC chromatogram also confirmed the inhibitoryactivities of PHAs against SSAO or ACE.Keywords: Angiotensin converting enzyme (ACE); degree of esterification (DE); semicarbazide-sensitiveamine oxidase (SSAO); pectin; pectin hydroxamic acid (PHA)
DE25T4 DE94T4.However, the order of ACE inhibition was DE94T4 > DE94T18 DE65T4 > DE25T4. The SSAOactivity staining or ACE-hydrolyzed products on TLC chromatogram also confirmed the inhibitoryactivities of PHAs against SSAO or ACE.Keywords: Angiotensin converting enzyme (ACE); degree of esterification (DE); semicarbazide-sensitiveamine oxidase (SSAO); pectin; pectin hydroxamic acid (PHA)