Analysis of Glycan-Related Genes Expression and Glycan Profiles in Mice with Liver Fibrosis
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- 作者：HanJie Yu ; MinZhi Zhu ; YanNan Qin ; YaoGang Zhong ; Hua Yan ; Qi Wang ; HuiJie Bian ; Zheng Li
- 刊名：Journal of Proteome Research
- 出版年：2012
- 出版时间：November 2, 2012
- 年：2012
- 卷：11
- 期：11
- 页码：5277-5285
- 全文大小：446K
- 年卷期：v.11,no.11(November 2, 2012)
- ISSN：1535-3907
文摘
Protein glycosylation plays an important role in the pathogenesis and progression of various liver diseases. However, little is known about the precise alterations in protein glycosylation or the potential correlation between glycan-related genes expression and glycan profiles in liver fibrosis. The aim of the study was to investigate potential associations between glycan-related genes expression and glycan profiles to evaluate liver fibrosis in a mouse model. Analyses of glycan-related genes expression and glycan profiles were performed using oligonucleotide microarrays and lectin microarrays, respectively. Real-time PCR and Western blot were used to confirm any altered glycan-related genes expression levels and protein levels. Moreover, altered glycan patterns on the surface of hepatocytes were verified by lectin histochemistry. These results revealed that the mRNA levels of 10 glycan-related genes were significantly altered in fibrotic liver. Furthermore, we observed an increase in multivalent sialic acid, poly-LacNAc, sialyl-T-antigen, Fucose伪-1,3/6GlcNAc, and GalNAc伪1鈥?Gal in fibrotic liver specimens, whereas GlcNAc oligomers was decreased in fibrotic liver. Our findings indicated that the synthetic pathway of 鈥淭n antigen 鈫?T antigen (core-1) 鈫?sialyl-T antigen鈥?was activated for O-glycan during the process of liver fibrosis.