Real-Time Fluorometric Assay for Acetylcholinesterase Activity and Inhibitor Screening through the Pyrene Probe Monomer鈥揈xcimer Transition

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• 作者：Jian Chen ; Dongli Liao ; Yan Wang ; Huipeng Zhou ; Wenying Li ; Cong Yu
• 刊名：Organic Letters
• 出版年：2013
• 出版时间：May 3, 2013
• 年：2013
• 卷：15
• 期：9
• 页码：2132-2135
• 全文大小：249K
• 年卷期：v.15,no.9(May 3, 2013)
• ISSN：1523-7052

文摘

A choline labeled pyrene probe (Py-Ch) was designed and synthesized. Poly(vinylsulfonate) (PVS) could induce Py-Ch aggregation. The aggregation and deaggregation process could be finely controlled by the acetylcholinesterase (AChE) enzymatic hydrolysis of Py-Ch. The resulting excimer鈥搈onomer transition provided a facile way for real-time AChE activity fluorometric assay and inhibitor screening.