Chemiluminescence Detection of a Protein through the Aptamer-Controlled Catalysis of a Porphyrin Probe

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• 作者：Wenying Li ; Qingfeng Zhang ; Huipeng Zhou ; Jian Chen ; Yongxin Li ; Cuiyun Zhang ; Cong Yu
• 刊名：Analytical Chemistry
• 出版年：2015
• 出版时间：August 18, 2015
• 年：2015
• 卷：87
• 期：16
• 页码：8336-8341
• 全文大小：387K
• ISSN：1520-6882

文摘

Sensitive and selective protein detection based on the aptamer-controlled noncovalent porphyrin probe self-assembly is reported for the first time. Vascular endothelial growth factor (VEGF) is a predominant biomarker in cancer angiogenesis. In this work, a positively charged porphyrin probe, manganese(III) meso-tetrakis(N-methylpyridinum-4-yl)porphyrin (Mn鈥揚yP), was prepared. Using it as a catalyst, a label-free chemiluminescence (CL) turn-on approach for sensitive VEGF detection is developed. Mn鈥揚yP could catalyze the luminol CL reaction. The VEGF aptamer could induce aggregation of Mn鈥揚yP. As a result, the Mn鈥揚yP-catalyzed CL reaction is efficiently suppressed. Upon the addition of VEGF, the specific binding of VEGF to the aptamer weakens the interactions between the aptamer and Mn鈥揚yP. The Mn鈥揚yP monomers are released, and a turn-on CL signal is thus detected. Our method is quite sensitive; 50 pM of VEGF could be easily detected. It is also very selective against other proteins. Our assay provides an aptamer-based efficient way for protein quantification.