Easy Ambient Sonic-Spray Ionization Mass Spectrometry Combined with Thin-Layer Chromatography

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• 作者：Renato Haddad ; Humberto M. S. Milagre ; Rodrigo Ramos Catharino ; Marcos N. Eberlin
• 刊名：Analytical Chemistry
• 出版年：2008
• 出版时间：April 15, 2008
• 年：2008
• 卷：80
• 期：8
• 页码：2744 - 2750
• 全文大小：547K
• 年卷期：v.80,no.8(April 15, 2008)
• ISSN：1520-6882

文摘

On-spot detection and analyte characterization on thin-layer chromatography (TLC) plates is performed via ambient desorption/ionization and (tandem) mass spectrometry detection, that is, via easy ambient sonic sprayionization mass spectrometry (EASI-MS). As proof-of-principle cases, mixtures of semipolar nitrogenated compounds as well as pharmaceutical drugs and vegetable oilshave been tested. The technique has also been applied tomonitor a chemical reaction of synthetic importance. EASIis the simplest and gentlest ambient ionization techniquecurrently available, assisted solely by N₂ (or air). It usesno voltages, no electrical discharges; no UV or laserbeams, and no high temperature and is most easilyimplemented in all API mass spectrometers. TLC is alsothe simplest, fastest, and most easily performed chromatographic technique. TLC plus EASI-MS thereforeprovide a simple and advantageous combination of chromatographic separation and sensitive detection of the TLCspots as well as on-spot MS or MS/MS characterization.The favorable characteristics of TLC-EASI-MS indicateadvantageous applications in several areas such as drugand oil analysis, phytochemistry and synthetic chemistry,forensics via reliable counterfeit detection, and qualitycontrol.