Asymmetric Bioreduction of Ethyl 3-Halo-2-oxo-4-phenylbutanoate by Saccharomyces cerevisiae Immobilized in Ca-Alginate Beads with Double Gel Layer

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• 作者：Humberto M. S. Milagre ; Cí ; ntia D. F. Milagre ; Paulo J. S. Moran ; Maria Helena A. Santana ; and J. Augusto R. Rodrigues
• 刊名：Organic Process Research & Development
• 出版年：2006
• 出版时间：May 2006
• 年：2006
• 卷：10
• 期：3
• 页码：611 - 617
• 全文大小：147K
• 年卷期：v.10,no.3(May 2006)
• ISSN：1520-586X

文摘

The asymmetric bioreduction of ethyl 3-halo-2-oxo-4-phenylbutanoate was studied for several microorganisms and especiallyfor Saccharomyces cerevisiae. The highest chemical yield (90%),de (70%) and ee (96-99%) were obtained with S. cerevisiaeimmobilized in calcium alginate beads with double gel layers,and reaction conditions were optimized by changing matrix ofimmobilization, concentration of substrate, and feeding withglucose as electron donor. The entrapment of cells with doublegel layers was fundamental to achieve high enantio- anddiastereoselectivity.